UBASH3A and TIGIT genes expression levels in systemic sclerosis … Original Research Article … |
The Egyptian Journal of Immunology E-ISSN (2090-2506) Volume 32 (2), April 2025 Pages: 92–101 www.Ejimmunology.org https://doi.org/10.55133/eji.320209 |
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| Aml A. R. Mohammed1, Fatma S. Abd-Elsamea2, Maha S. I. Abdelrahman3, Fatma M. A. Mohamed4, Fatma Y. A. Abbas5, and Fatma M. Helbawi1 |
| 1Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.
2Department of Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. 3Department of Rheumatology, Rehabilitation & Physical Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt. 4Department of Histology, Faculty of Medicine, Assiut University, Assiut, Egypt. 5Department of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt.
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Corresponding author: Fatma S. Abd-Elsamea, Department of Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. Email: fatmasayed@aun.edu.eg. |
Abstract
Systemic sclerosis (SSc) is an autoimmune disorder marked by excessive fibrosis, microvascular stenosis, and systemic clinical manifestations. An autoimmune process is believed to induce T-cell activation, mainly CD 4 T helper cells, and enhance production of proinflammatory and profibrotic cytokines such as interleukin (IL) 4 and IL 13. These cytokines contribute to vasculopathy and excessive collagen synthesis. UBASH3a (Ubiquitin Associated and SH3 Domain Containing A) and TIGIT (T cell immunoglobulin and ITIM domain) have an important role in limiting unwarranted T cell activation and maintaining immune tolerance. Our study aimed to explore UBASH3A and TIGIT mRNA expression levels in 30 SSc patients compared to 30 age and sex matched normal controls. We measured mRNA levels via real-time quantitative reverse transcription polymerase chain reaction in total RNA isolated from the peripheral blood mononuclear cells (PBMCs) of SSc patients and controls. We used RNA extraction commercial kits. The expression levels of UBASH3A and TIGIT mRNA were significantly higher in PBMCs from SSc patients compared to control subjects.
Keywords:
UBASH3A, TIGIT, T cell, Systemic Sclerosis
Date received: 29 October 2024; accepted: 22 April 2025
PMID:
40285746
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