Transcription factor 7 like 2 gene polymorphism and advanced glycation end products as risk factors for diabetic nephropathy….. Original Research Article….. |
The Egyptian Journal of Immunology E-ISSN (2090-2506) Volume 31 (3), July, 2024 Pages: 01–14. www.Ejimmunology.org https://doi.org/10.55133/eji.310301 |
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| Madeeha Y. Bakheet¹, Ebtsam F. EL-Karn¹, Omnia A. Mohamed¹, Ghadeer Abdel Razzak¹, Eman M. Abdelrahman¹, and Walaa A. Khalifa² |
| Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Internal Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt. |
Corresponding author: Ghadeer Abdel Razzak, Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt. Email: ghadeer.razzak111@hotmail.com |
Abstract
Diabetic nephropathy (DN) is the most serious and prevalent complication, being a major contributing factor to end-stage renal disease, that can lead to death in diabetic patients. The main pathologic changes of DN are the accumulation of advanced glycation end products and growth factors. Genetic variations may have an impact on the initiation and development of DN. Transcription factor 7 like 2 (TCF7L2) gene may contribute to the etiology of DN in combination with other genes. We aimed to determine whether the genotype distribution of single nucleotide polymorphism rs7903146 of TCF7L2 gene and level of advanced glycation end products (AGEs) could be risk factors for DN. The study included 54 diabetic patients, classified as 36 diabetic patients with nephropathy and 18 diabetic patients without nephropathy. The frequency of genotypes of SNP rs7903146 of TCF7L2 gene in diabetic patients without nephropathy was CC: 50%, CT: 33.3% and TT: 16.7%, while in diabetic patients with nephropathy was CC: 16.7%, CT: 69.4% and TT: 13.9%. In diabetic patients with nephropathy, risk allele carrier genotypes (CT+TT) represented 83.3% compared to 50% in diabetic patients without nephropathy. The frequency of allele distributions of SNP rs7903146 C/T of TCF7L2 gene in diabetic patients without nephropathy was C allele: 66.7% and T allele: 33.3%, while in diabetic patients with nephropathy was C allele: 51.4% and T allele: 48.6%. The T allele of SNP rs7903146 C/T of TCF7L2 gene was predominant in diabetic patients with nephropathy. Also, AGEs were significantly elevated in diabetic patients with nephropathy. In conclusion, the T allele of SNP rs7903146 C/T of TCF7L2 gene and risk allele carrier genotypes (CT+TT) were associated with the susceptibility to development of nephropathy in type 2 diabetes mellitus patients. AGEs could be a good biomarker in monitoring progression of DN disease.
Keywords:
Diabetic nephropathy, Transcription factor 7 like 2, Advanced glycated end products.
Date received:10 October 2023; accepted: 22 April 2024
PMID:
38985530
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