Disheveled EGL-10 and pleckstrin domain-containing 5 rs1012068 T/G gene polymorphism among Egyptian chronic HCV-infected patients: disease progression and related complications |
The Egyptian Journal of Immunology Volume 29 (3), July, 2022 Pages: 36–43. www.Ejimmunology.org https://doi.org/10.55133/eji.290305 |
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| Hanan M. Farhan1, Khadiga Abougabal1, Heba F. Gaber1 and Dina Attia2
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1Department of Clinical & Chemical Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
2Department of Tropical Medicine, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
| Corresponding author: Hanan M. Farhan, Department of Clinical & Chemical Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt. Email: hananmohamedfarhan@gmail.com. |
Abstract
Hepatitis C virus (HCV) infection related complications including fibrosis, cirrhosis and hepatocellular carcinoma (HCC) are influenced by host genetic factors. Identification of emerging host genetic variations is of promising value. Disheveled EGL-10 and pleckstrin domain-containing 5 (DEPDC5) rs1012068 T/G gene polymorphism has been implicated in liver disease. This study aimed to assess DEPDC5 rs1012068 T/G gene polymorphism with disease progression and related complications among Egyptian patients with chronic HCV infection. Sixty chronic HCV-infected patients and 60 apparently healthy controls were recruited in this study. Patients were classified into 20 with liver fibrosis, 20 with liver cirrhosis and 20 with HCC; all recruited from Outpatients Clinic and Tropical Medicine Inpatient Department, Faculty of Medicine, Beni-Suef University Hospital. DEPDC5 rs1012068 T/G gene polymorphism was assayed by real time-polymerase chain reaction (RT-PCR) TaqMan allelic discrimination. DEPDC5 rs1012068 GG genotype and G allele variants showed statistically significant higher frequency among patients with liver fibrosis when compared to controls (OR (95% CI) 10.500 (2.086 – 52.851), P= 0.004 and 0.388 (0.155 – 0.971), P= 0.011), respectively. DEPDC5 rs1012068G allele variant showed statistically significant higher frequency among patients with liver fibrosis when compared to HCC patients (OR (95% CI) 3.316 (1.286 – 8.550), P= 0.012) and to both HCC and cirrhosis patients (OR (95% CI) 2.579 (1.187-5.645), P= 0.016). In conclusion, our results suggest that DEPDC5 rs1012068 G allele could be considered genetic risk allele for liver fibrosis and disease progression among Egyptian patients with chronic HCV infection.
Keywords: DC5 rs1012068 T/G, hepatitis C virus, real-time polymerase chain reaction (RT-PCR), liver fibrosis.
Date received: 17 February 2022; accepted: 22 April 2022
PMID: 35758967
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