Study of transforming growth factor beta 1 gene +869T/C polymorphism in Egyptian rheumatoid arthritis patients and its relation to disease activity

The Egyptian Journal of Immunology
Volume 29 (1), January, 2022
Pages: 19–28.
www.Ejimmunology.org
https://doi.org/10.55133/eji.290103
Asmaa S. Hassan1, Abeer M. Abdul-Mohymen1, Laila A. Ahmed2, Nora Seliem3, Marwa M. R. Hassan4, Hanan F. Ibrahim5, Mona G. Al Anan6
1Clinical Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.

2Internal Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.

3Biochemistry Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.

4Community Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.

5Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.

6Physiology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.

Corresponding author: Asmaa S. Hassan, Clinical Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Email: asmsobhy@gmail.com.

Abstract

This study intended to explore the relationship between the +869T/C polymorphism of the transforming growth factor-β1 (TGF-β1) gene and rheumatoid arthritis (RA) predisposition and activity in Egyptian patients. The study involved 30 patients suffering from RA and 30 apparently healthy participants as the control group. The +869T/C polymorphism of the TGF-β1 gene was determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) process. The TGF-β1 + 869 CT genotype and CT+TT genotypes in RA patients showed a significant increase than the control group (OR=3.782 and 3.824, CI=1.046-13.680 and 1.150-12.713, P=0.043 and 0.029, respectively). T allele showed a significant increase in patients than in controls (OR= 2.104, CI 1.015- 4.361, P = 0.046). The TGF-β1 +869 CT+TT genotypes were accompanied by higher DAS-28 scores which express higher disease activity, and increased levels of RF, Anti-CCP, ESR, and CRP. In conclusion, the TGF-β1 +869T/C gene polymorphism may be accompanied by an increased predisposition to RA and with its severity in Egyptian RA patients.

Keywords: TGF-β1, +869T/C, Polymorphism, Rheumatoid Arthritis

Date received: 23 October 2021; accepted: 22 December 2021

PMID: 35171544

 

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