Evaluation of stromal cell-derived factor-1β Gene polymorphism G801A among Egyptian patients with type 2 diabetes mellitus

The Egyptian Journal of Immunology
Volume 28 (3), July, 2021
Pages: 94–101.
www.Ejimmunology.org
https://doi.org/10.55133/eji.280310
Hanan M Farhan1, Khadiga Abougabal1, Saly A Abd Ellatif1, Rania E Sheir2
1Department of Clinical & Chemical Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.

2Department of Internal Medicine, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.

Corresponding author: Hanan M Farhan, Department of Clinical & Chemical Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
Email: hananmohamedfarhan@gmail.com

Abstract

Stromal cell derived factor – 1 (SDF-1) is expressed by bone marrow derived stromal cells and stromal tissues in multiple organs. SDF-1 possesses diverse physiological and biochemical functions concerning autoimmunity and inflammation. SDF-1 gene polymorphisms play various roles in immune related diseases. This study aimed to evaluate association of SDF-1β G801A gene polymorphism and type 2 diabetes mellitus (T2DM) as regard prevalence and glycemic control. The study included hundred Egyptian subjects; 50 patients with T2DM recruited from the Internal Medicine Department, Beni-Suef University Hospital, and 50 age and sex matched healthy subjects. Participants were subjected to full clinical examination and routine laboratory investigations including fasting blood glucose, 2 hours post prandial blood glucose, serum creatinine, lipid profile and glycated hemoglobin (HbA1c). SDF-1β G801A gene polymorphism was analyzed using polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP). The frequency of GG and AG genotypes was higher in patients compared to controls with no statistical significance. Higher frequency of homozygous GG genotype and G allele were observed among patients with poor glycemic control (HbA1c ≥ 8% ) compared to those with good glycemic control (HbA1c < 8%) (P = 0.001 and 0.018 respectively). In conclusion, this study suggested an association between SDF-1 β gene polymorphism and type 2 diabetes mellitus in Egyptian patients. Further studies are needed to confirm our observations.

Keywords: Stromal cell derived factor – 1 (SDF-1), gene polymorphism, type 2 diabetes mellitus (T2DM), polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP)

Date received: 23 January 2021; accepted: 22 April 2021

PMID: 34185458

 

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